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4 6 diamidino 2 phenylindole dapi  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology 4 6 diamidino 2 phenylindole dapi
    Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were <t>stained</t> with <t>4′,6-diamidino-2-phenylindole</t> (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
    4 6 Diamidino 2 Phenylindole Dapi, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+reagent/DAPI+Reagent/pmc13683952-441-9-12
    Average 94 stars, based on 31 article reviews
    4 6 diamidino 2 phenylindole dapi - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Inverse Vaccine Nanomimics: In situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post-Myocardial Infarction"

    Article Title: Inverse Vaccine Nanomimics: In situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post-Myocardial Infarction

    Journal: Research

    doi: 10.34133/research.1464

    Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
    Figure Legend Snippet: Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Techniques Used: Immunofluorescence, Control, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Tissue

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    Image Search Results


    Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Journal: Research

    Article Title: Inverse Vaccine Nanomimics: In situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post-Myocardial Infarction

    doi: 10.34133/research.1464

    Figure Lengend Snippet: Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Article Snippet: For T cell immunofluorescence labeling, nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (E-CK-A163, Elabscience, China), CD4 with FITC (E-AB-F1097C, Elabscience, China), and CD127 with Alexa Fluor 555 (16-1271-85, eBioscience, USA).

    Techniques: Immunofluorescence, Control, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Tissue